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Serum antigens of cattle. VI. Isolation and properties of an intracellular antigen (Eel) from cattle erythrocytes *
Author(s) -
Bhargava G.,
Stone W. H.
Publication year - 1977
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1977.tb01648.x
Subject(s) - sephadex , antigen , size exclusion chromatography , periodate , isoelectric focusing , isoelectric point , biochemistry , polyacrylamide gel electrophoresis , biology , microbiology and biotechnology , hemoglobin , chemistry , polyacrylamide , chromatography , enzyme , immunology
Summary The erythrocyte antigen (Ecl) of cattle has been isolated using DEAE‐cellulose chromatography, Sephadex gel filtration, and isoelectrofocusing. The purified antigen is free from hemoglobin and migrates as a single protein on polyacrylamide gels. It exhibits an absorption maximum at 278 nm and has a molecular weight of about 100 000; its isoelectric point is 4.95. The antigen has a carbohydrate moeity and loses its activity after oxidation with periodate. The evidence suggests that it is not a common isozyme, but its exact identity remains unknown.