Premium
A new hemoglobin β chain variant in sheep
Author(s) -
John M. E.,
John Manorama
Publication year - 1977
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1977.tb01645.x
Subject(s) - hemoglobin , urea , biology , allele , microbiology and biotechnology , hemoglobin variants , biochemistry , electrophoresis , gene , beta (programming language) , genetics , chemistry , computer science , programming language
Summary A new hemoglobin β chain variant referred to as Hb‐E is observed in four Indian sheep breeds. Hemoglobin E is electrophoretically and chromatographically un‐distinguishable from hemoglobin B. However, polypeptide chain separation by urea‐polyacrylamide disc gel electrophoresis and urea CM‐cellulose chromatography reveals the new β chain. The β E chain has a slower anodic mobility than £ B chain at alkaline pH. The adult sheep hemoglobins could be classified into eight categories, namely AA, AB, AE, AC, ABC, BB, BE and EE, according to their sub‐unit composition. The occurrence of β E chain in homozygous condition or in combination with either one of the two allelic β chains β A or β B , indicate that it may be an allelic variant. Gene frequencies of three hemoglobin β chain alleles observed during the study are as follows: A = 0.192; B = 0.682; E = 0.126.