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Horizontal polyacrylamide gradient gel electrophoresis for the simultaneous phenotyping of transferrin, post‐transferrin, albumin and post‐albumin in the blood plasma of cattle
Author(s) -
Gahne Bo,
Juneja R. Kumar,
Grolmus Jan
Publication year - 1977
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1977.tb01637.x
Subject(s) - transferrin , albumin , polyacrylamide gel electrophoresis , chromatography , biology , allele , gel electrophoresis , microbiology and biotechnology , biochemistry , chemistry , gene , enzyme
Summary A simple method of horizontal polyacrylamide gel electrophoresis was described for the simultaneous phenotyping of transferrin, post‐transferrin, albumin and post‐albumin in the blood plasma of cattle. A step gradient gel of 8, 4, 12 and 14 % acrylamide concentration was used. The method enabled the detection of a new protein polymorphism in the post‐transferrin region. Two alleles were observed. The transferrin phenotypes involving D 1 and D 2 alleles were clearly separated. The resolution of the post‐albumin fractions was also better than described by earlier methods.