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Characterization of chicken plasma alkaline phosphatase isozymes by urea and heat treatments
Author(s) -
Tamaki Yoshinori,
Morichi Toshiki
Publication year - 1976
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1976.tb01392.x
Subject(s) - urea , isozyme , alkaline phosphatase , enzyme , biochemistry , biology , chemistry , microbiology and biotechnology
Summary The effects of urea and heat treatments on electrophoretic pattern and activity were investigated in chicken plasma alkaline phosphatase (AP). The B band, which had a slower migrating rate to the F or S band irrespective of isozyme types, was labile to urea (4M) and heat treatments (60 o C, 10 min), while the F and S bands were stable to the same treatments. From these results, the genetic control of the three chicken plasma AP isozymes, i.e., F, S and B bands, was discussed. The total AP activity of the F or S type was little affected by urea treatment in spite of the unstableness of the B band. It is considered that the B band inactivated by urea restores the activity when the urea concentration was reduced. The AP activity was reduced by the heat treatment. The reduction may be primarily due to the loss of the activity of the B band.