Premium
Immunogenetic polymorphism of lipoproteins in swine. 1. Four additional serum β‐lipoprotein allotypes (Lpp2, Lpp4, Lpp5 and Lpp15) in the Lpp system 1
Author(s) -
Rapacz J.,
HaslerRapacz Judith,
Kuo W. H.,
Li D.
Publication year - 1976
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1976.tb01391.x
Subject(s) - biology , allotype , allele , immunodiffusion , genetics , lipoprotein , genotype , gene , radial immunodiffusion , antigen , phenotype , antibody , cholesterol , biochemistry
Summary Four additional swine serum lipoprotein allotypes are described. Specific anti‐allotype reagents were obtained from alloimmune precipitating sera produced in lipoprotein‐defined‐type recipients immunized with normal sera and subsequently with lipoprotein fractions. Identification studies indicate that the four serologically defined low‐density lipoprotein (LDL) variants, designated Lpp2, Lpp4, Lpp5 and Lpp15, are members of a previously described Lpp system. The individual specificities, Lpp2, Lpp4 and Lpp5, are determined by three co‐dominant autosomal genes, Lpp 2 , Lpp 4 and Lpp 5 , respectively, whereas the common specificity, Lpp 15, is controlled by a complex of genetic information of the Lpp‐ and Lpp* genes, and by the two previously described alleles, Lpp 1 and Lpp 3 ; Lpp15 occurs on the same molecule with respective individual specificity. The Lpp5 and Lpp15 antigens behave as a pair of alternative allotypic specificities. The double immunodiffusion test in agar was employed to demonstrate independent phenotypic expression of each allelic gene in the Lpp heterozygous animals, for the analysis of the immune sera, and for lipoprotein testing of 3305 sera. Marked differences in gene frequencies were found between the swine breeds tested. As a result of characteristic frequencies, only nine of 15 possible Lpp genotypes were found in the breeding herds tested; the remaining six genotypes were obtained from testcross matings.