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Genetic variation in the purine nucleoside phosphorylase activity of sheep red cells
Author(s) -
Tucker E. M.,
Young J. D.
Publication year - 1976
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1976.tb01384.x
Subject(s) - purine nucleoside phosphorylase , biology , purine , red cell , enzyme , biochemistry , gene , purine metabolism , glycogen phosphorylase , isozyme , nucleoside , nucleotide salvage , microbiology and biotechnology , nucleotide , medicine
Summary The purine nucleoside phosphorylase (NP) activity of sheep red cells was determined by starch gel electrophoresis and by a spectrophotometric assay technique. Some sheep had high activity (NP‐high type) and some had low or zero activity (NP‐low type). The enzyme deficiency is apparently confined to the red cell since other tissues from NP‐low type animals had activities similar to those from NP‐high type individuals. Family data indicated that NP activity is controlled by a pair of autosomal allelic genes, designated NP H and NP L . Sheep heterozygous for the NP genes had lower enzymic activities than homozygous high‐type individuals. The frequency of NP types in different breeds of sheep was determined. Barbary and Mouflon sheep had activities similar to NP‐high type domestic sheep; goats had high enzyme activities but their NP had a slower electrophoretic mobility than that of sheep.