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J blood group active lipoproteins extracted from bovine erythrocytes
Author(s) -
Kirchhof Bruno,
Thiele Otto Wolfgang,
Petri Maria Elisabeth,
Koch Johannes
Publication year - 1976
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1976.tb01378.x
Subject(s) - centrifugation , ultracentrifuge , chromatography , lipoprotein , dextran , chemistry , fraction (chemistry) , analytical ultracentrifugation , membrane , biochemistry , cholesterol
Summary Ghosts of J‐positive bovine erythrocytes were frozen overnight, thawed the next day, washed with ion‐free water, and then extracted with a water‐n‐pentanol mixture. After centrifugation, the aqueous phase contained a great deal of the membrane proteins and lipids in a soluble form along with the J blood‐group activity. After preparative ultracentrifugation, about 2/3 of the J activity were recovered in the high‐density lipoprotein fraction, while the low‐density lipoprotein fraction contained 1/3 J activity. This result is consistent with our finding that on treatment of the stroma lipoproteins with dextran sulphate, about 2/3 of J activity were recovered in the supernatant, 1/3 in the precipitate. These fractions obtained by dextran sulphate treatment were characterized by protein and lipid assay.