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Agglutinability of cattle red cells. 4. The effect of antiglobulin in comparison with treatment by pronase
Author(s) -
Sellei J.
Publication year - 1975
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1975.tb01348.x
Subject(s) - pronase , biology , immunology , biochemistry , trypsin , enzyme
Summary The average titre scores varied from zero to 24.7 for the cattle red cells (CRC) from different MZ pairs. These CRC were sensitized with different blood‐typing reagents and were titre‐tested against the double dilution series of an anti‐bovine y‐globulin serum. A significant negative correlation (r = ‐ 0.82; P <0.001) was found between the degree of agglutinability and the amount of neuraminic acid of the surface component of CRC cleaved by pronase. After pronase treatment of the CRC it could be demonstrated that (1) the activity of the V and E' 3 blood factors became destroyed; (2) three new specific receptors became evolved; (3) the degree of ‘direct’ agglutinability due to the A 2 , O 3 W, S 2 and Z anti‐sera did not parallel with the titre scores obtained in the anti‐globulin tests.