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Agglutinability of cattle red cells. 2. Agglutination by isologous and heterologous sera of enzyme‐treated red cells
Author(s) -
Sellei J.
Publication year - 1974
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1974.tb01335.x
Subject(s) - agglutination (biology) , heterologous , neuraminidase , biology , trypsin , homologous chromosome , microbiology and biotechnology , enzyme , immunology , antibody , biochemistry , gene
Summary Neuraminidase treatment made cattle red cells (CRC) agglutinable by the agglutinins of the different heterologous but not of the homologous sera. These agglutinins were, however, absorbed by both the nauraminidase‐treated and the intact CRC. Proteolytic treatment made CRC agglutinable also by the normal cattle, isoimmune and autologous sera. Agglutination titres of the CRC ranged from 1: 2 to 1: 256, but the variation between CRC from members of monozygous (MZ) pairs was not greater than ± 2 agglutination score units the range of experimental error. Treatment with trypsin made the A 1 , A 2 factors more emergent on the surface of CRC for agglutination by anti‐A 2 , while pronase treatment had a similar effect upon agglutination of Z‐positive cells by anti‐Z.