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Agglutinability of cattle red cells. 1. Agglutination by lectins of enzyme‐treated red cells
Author(s) -
Sellei J.
Publication year - 1974
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1974.tb01334.x
Subject(s) - pronase , trypsin , titer , biology , agglutinin , agglutination (biology) , lectin , microbiology and biotechnology , chymotrypsin , enzyme , proteolytic enzymes , biochemistry , immunology , antigen , antibody
Summary Pronase‐treated cattle red cells (CRC) from different monozygous (MZ) twin pairs could be classified as weakly (titre 1: 2, 1: 4) or strongly (titre 1: 1024, 1: 4096) agglutinable when Phytohemagglutinin‐M (Phy‐M) was used as agglutinin. In the presence of concavalin‐A (Con‐A), the CRC from different MZ pairs treated with pronase, A‐chymotrypsin or trypsin showed a gradation from low (titre 1: 32) to high (titre 1: 256 000) agglutinability. The trypsin‐treated CRC which had A 1 , A 2 blood factors usually had a titre of 1: 8000 or higher with Con‐A. Both the intact CRC and the CRC treated with proteolytic enzymes were capable of absorbing the Phy‐M or Con‐A lectins.