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Simultaneous electrophoretic analysis of a large number of samples. Application to serum esterases of Taterillus (rodent)
Author(s) -
Baron J. C.
Publication year - 1973
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1973.tb01269.x
Subject(s) - citation , rodent , library science , microbiology and biotechnology , computer science , humanities , biology , philosophy , ecology
Genetical and biocheniical studies have increasingly used electrophoretic techniques these last ten years and it is more specially in population genetics that analysis of a large number of samples is needed. An apparatus has been developed which permits the analysis of as many as 40 samples in the same starch gel run according to Smithies’ technics (1 955). This apparatus which has analogies with Manuel’s (Creyssel, 1968) has already been described (Baron, 1972) and tested with more than 1 O00 sera. The 40 samples, migrating under identical electrophoretical conditions (without any distortion of the front), are directly comparable and can be submitted to three different treatments on the three slices of the gel. Fig. 1 illustrates the results obtained with 35 Taterillus sera after starch gel electrophoresis and esterase staining according to the procedure of Baron (1972). On the base of electrophoretic mobility and differential substrate (acetyl or propionyl) and inhibitor (dichlorvos, eserine, p-CMB) specifity, the esterases can be divided ‘into 5 groups (Table 1).