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Haemolytic factor in bovine seminal vesicle fluid
Author(s) -
Matoušek J.,
Staněk R.
Publication year - 1970
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1970.tb01154.x
Subject(s) - pronase , sephadex , chymotrypsin , trypsin , chromatography , enzyme , biology , vesicle , chemistry , biochemistry , membrane
Summary The haemolytic factor of bovine seminal fluid has a protein nature; it is present in the precipitate obtained by treating the fluid with alcohol‐ether, ammonium sulphate and rivanol; it occurs in three out of five protein fractions obtained by chromatographic separation on Sephadex G 100. of Enzymatic treatment of seminal vesicle fluid by pronase caused total inactivation of the haemolytic factor. Chymotrypsin caused considerable damage, while a number other enzymes did not affect the activity of the haemolytic factor.