Premium
Vitamin D3 treatment of Crohn’s disease patients increases stimulated T cell IL‐6 production and proliferation
Author(s) -
BendixStruve M.,
Bartels L. E.,
Agnholt J.,
Dige A.,
Jørgensen S. P.,
Dahlerup J. F.
Publication year - 2010
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.2010.04463.x
Subject(s) - medicine , vitamin d and neurology , vitamin , peripheral blood mononuclear cell , placebo , cholecalciferol , t cell , crohn's disease , cytokine , immune system , gastroenterology , calcitriol , immunology , endocrinology , in vitro , pathology , disease , biology , biochemistry , alternative medicine
Aliment Pharmacol Ther 2010; 32: 1364–1372 Summary Background Vitamin D3 has shown immune‐modulating effects in CD4+ T cells from Crohn’s disease patients in vitro . Aim To investigate the effects of in vivo vitamin D3 treatment on T cells in Crohn’s disease patients. Methods Peripheral blood mononuclear cells (PBMC) were isolated at week 0 and at week 26 from 10 vitamin D3‐ and 10 placebo‐treated Crohn’s disease patients participating in a randomized, placebo‐controlled, clinical trial study. Monocyte‐depleted PBMC were stimulated with anti‐CD3 and anti‐CD28, and cultured for 7, days, to investigate CD4+ T‐cell proliferation and T‐cell cytokine production. Results In vitamin D3‐treated patients, the median 25‐hydroxyvitamin D3 levels increased 70 nmol/L compared with −5 nmol/L in the placebo group. Vitamin D3 treatment increased interleukin‐6 production (delta = 188 pg/mL, range: −444 to 4071) compared with a decrease in the placebo group (delta = −896 pg/mL, range: −3841 to 1323) ( P < 0.02, Wilcoxon rank sum test). Interestingly, vitamin D3 increased the amount of proliferating stimulated CD4+ T cells from median 41% (range: 10–75%) to 56% (range: 26–77%) ( P = 0.02, Wilcoxon rank sum test). Conclusions Vitamin D3 treatment of Crohn’s disease patients increased the IL‐6 levels. Interestingly, vitamin D3 treatment enhanced the CD4+ T cell proliferation.