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Assessment of antimicrobial microspheres as a prospective novel treatment targeted towards the repair of perianal fistulae
Author(s) -
BLAKER J. J.,
PRATTEN J.,
READY D.,
KNOWLES J. C.,
FORBES A.,
DAY R. M.
Publication year - 2008
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.2008.03773.x
Subject(s) - metronidazole , bacteroides fragilis , antimicrobial , microbiology and biotechnology , microsphere , staphylococcus aureus , antibiotics , medicine , drug delivery , escherichia coli , bacteria , chemistry , nanotechnology , biochemistry , materials science , biology , chemical engineering , gene , genetics , engineering
Summary Background None of the proposed materials tested for the management of perianal fistulae has proven to be a definitive treatment. Aim To assess a new repair scaffold and drug delivery device conceived to target perianal fistula repair. Methods Poly(d,l‐lactide‐co‐glycolide) porous microspheres containing either antibacterial silver‐releasing degradable phosphate glass or metronidazole were prepared using thermally induced phase separation. Results Ion‐ and drug‐release profiling of the microspheres revealed continued release of silver ions from microspheres filled with silver‐doped phosphate glass and high encapsulation efficiency for metronidazole [78% and 82% for microspheres loaded with 2.5% and 1.3% (w/w), respectively]. Microbicidal activity was confirmed by growth inhibition of bacterial species ( Staphylococcus aureus , Escherichia coli and Bacteroides fragilis ), which characteristically dominate the colonization of perianal fistula tracts. Microspheres containing >3 mol% silver or metronidazole resulted in strong bacterial inhibition/kill against B. fragilis ; the presence of one sphere containing >3 mol% silver had a potent inhibitory effect against all the microbes studied. Microspheres became rapidly integrated with host tissue following subcutaneous implantation into a rodent wound model. Conclusion The study demonstrates a novel scaffold for guided tissue regeneration providing local release of antimicrobial agents sufficient to counter bacterial colonization and warrants further investigation.