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Evaluation of a new rapid immunoassay for the detection of Helicobacter pylori in faeces: a prospective pilot study
Author(s) -
Trevisani L.,
Sartori S.,
Rossi M. R.,
Ruina M.,
Matarese V.,
Gullini S.,
Abbasciano V.
Publication year - 2005
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.2005.02355.x
Subject(s) - helicobacter pylori , medicine , immunoassay , rapid urease test , gastroenterology , spirillaceae , feces , cytology , gold standard (test) , breath test , prospective cohort study , immunology , gastritis , antibody , pathology , microbiology and biotechnology , biology
Summary Background : Detection of Helicobacter pylori antigen in faeces is a valid method to diagnose H. pylori infection. Presently available stool tests are performed in the laboratory, and diagnostic report is delayed. Aim : To evaluate a new rapid stool test in a pre‐treatment setting and to compare it with a validated laboratory stool test. Methods : A total of 105 patients underwent gastroscopy with brush cytology, and biopsies for histology and rapid urease test, to assess H. pylori presence. Helicobacter pylori ‐status was considered positive if at least two tests were positive; negative if all tests were negative; indeterminate if one test was positive and two negative. Stool specimens were tested using either a rapid immunoassay kit (ImmunoCard STAT) or a laboratory enzyme immunoassay kit (Hp StAR). Results : Sixty patients were infected with H. pylori , 44 non‐infected, one indeterminate. The sensitivity and specificity of ImmunoCard STAT were 85 and 93%; those of Hp StAR were 88 and 100% (not significant). Conclusions : ImmunoCard STAT seems a reliable method for detecting H. pylori in untreated patients. It could replace laboratory stool tests, as it is easy and can be performed quickly. These characteristics might be a breakthrough for diagnosing H. pylori in the doctor's office.