Premium
Barrett's oesophagus with predominant intestinal metaplasia correlates with superficial cyclo‐oxygenase‐2 expression, increased proliferation and reduced apoptosis: changes that are partially reversed by non‐steroidal anti‐inflammatory drugs usage
Author(s) -
Amano Y.,
Ishihara S.,
Kushiyama Y.,
Yuki T.,
Takahashi Y.,
Chinuki D.,
Miyake T.,
Miyaoka Y.,
Rumi M. A. K.,
Ishimura N.,
Adachi K.,
Kinoshita Y.
Publication year - 2004
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.2004.02195.x
Subject(s) - epithelium , mucin , intestinal metaplasia , intestinal epithelium , pathology , apoptosis , stromal cell , proliferation index , medicine , cancer research , biology , dysplasia , microbiology and biotechnology , immunohistochemistry , biochemistry
Summary Background : Cyclo‐oxygenase‐2 expression has been reported to play an important role in the metaplasia‐dysplasia‐carcinoma sequence in Barrett's oesophagus. However, the existence of cyclo‐oxygenase‐2 expressing cells in Barrett's epithelium is still uncertain. Aim : To identify the cells that express cyclo‐oxygenase‐2 protein and to investigate the relationship between cyclo‐oxygenase‐2 expression and mucin‐phenotype of Barrett's epithelium. Methods : Sections from 466 biopsy samples of Barrett's epithelium from 358 non‐medicated patients were immunohistochemically examined for the cyclo‐oxygenase‐2 expression, mucin‐phenotype, cell proliferation and apoptosis. Results : Cyclo‐oxygenase‐2 expression was detected in 71.0% of Barrett's epithelium biopsy samples. In Barrett's epithelium with the gastric predominant mucin‐phenotype, cyclo‐oxygenase‐2 expression was mainly found in stromal and deep epithelial cells, whereas in intestinal predominant mucin‐phenotype, it was mostly in superficial epithelial cell. A significant elevation of proliferating cell nuclear antigen index and suppression of apoptotic index was observed in Barrett's epithelium with superficial epithelial cyclo‐oxygenase‐2 expression. Neither such elevation of proliferating cell nuclear antigen index nor the suppression of apoptotic index could be found in chronic non‐steroidal anti‐inflammatory drugs users. Conclusions : Barrett's epithelium with intestinal mucin and superficial epithelial cyclo‐oxygenase‐2 expression possess a higher proliferation potential, but this risk may be thwarted by non‐steroidal anti‐inflammatory drugs administration.