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Diagnostic value of culture, histology and PCR for Helicobacter pylori in the remnant stomach after surgery
Author(s) -
Matsukura N.,
Tajiri T.,
Kato S.,
Togashi A.,
Masuda G.,
Tokunaga A.,
Yamada N.
Publication year - 2004
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.2004.01989.x
Subject(s) - stomach , histology , helicobacter pylori , medicine , gastroenterology , biopsy , gastritis , microbiological culture , pathology , biology , bacteria , genetics
Summary Background : Diagnosis of Helicobacter pylori infection in the remnant stomach has not been established. Aims : To investigate the diagnostic value of culture, histology, PCR and serum IgG against H. pylori (ELISA) with and without eradication therapy in the remnant stomach, compared with the unoperated stomach. Methods : Biopsy samples for bacterial culture and histological diagnosis of H. pylori were taken from the stoma and upper corpus of the remnant stomach and gastric juice was used for PCR assay. Results : Bacterial culture‐based diagnosis in the remnant stomach, sensitivity and specificity of culture were 95.1%, 100%; histology 89%, 92.3%; PCR 66%, 89.7%; and ELISA 100%, 50%, respectively, in cases without H. pylori eradication therapy. In assessment of the results of therapy for the remnant stomach, sensitivity and specificity of culture were 100%, 100%; histology 80%, 96.8%; PCR 80%, 91.7%; and ELISA 100%, 0%, respectively. Conclusion : Bacterial culture had the highest diagnostic value in the remnant stomach as well as unoperated stomach. Sensitivity by histology and PCR was lower in the remnant stomach than the unoperated stomach, but specificity values were equal. Serum ELISA assay was not suitable for the remnant stomach.