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Analysis of the cag pathogenicity island and IS605 of Helicobacter pylori strains isolated from patients with gastric cancer in Japan
Author(s) -
Deguchi R.,
Igarashi M.,
Watanabe K.,
Takagi A.
Publication year - 2004
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.2004.01982.x
Subject(s) - caga , helicobacter pylori , pathogenicity island , cancer , antibody , gene , spirillaceae , medicine , pathogenicity , biology , microbiology and biotechnology , gastroenterology , immunology , virulence , genetics , gastritis
Summary Background : CagA protein is encoded by the cag A gene, which is part of the cag pathogenicity island (PAI) in Helicobacter pylori . Insertion sequence (IS) elements are a diverse set of specialized DNA segments that can move to new sites in bacterial genomes. Aim : To determine the role of cag PAI and IS 605 in the development of gastric cancer, we analysed cag PAI from patients with gastric cancer and compared the results with the host's CagA antibody status. Methods : H. pylori strains were isolated from 29 gastric cancer patients, and CagA status was determined by measuring serum antibody against CagA. The cag PAI region and IS 605 were determined by PCR. Results : CagA seropositivity tended to be higher in the IS 605 /PAI + group (5/7, 71.4%) than in the IS 605 /PAI – group (9/22, 40.9%). Association with cag13 was more frequent in the IS 605 + group (92.3%; 12/13) than in the IS 605 – group (25.0%; 4/16; P = 0.0005). Conclusions : cag 13 may be associated with the presence of IS 605 in gastric cancer patients.