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Review article: Pathobiology of neutrophil interactions with intestinal epithelia
Author(s) -
MADARA J. L.
Publication year - 1997
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.1997.tb00809.x
Subject(s) - paracellular transport , secretion , epithelium , in vivo , in vitro , intestinal epithelium , microbiology and biotechnology , immunology , caco 2 , integrin , cell , biology , medicine , pathology , membrane , biochemistry , permeability (electromagnetism)
SUMMARY Neutrophil‐epithelial interactions were modelled using polarized T84 cells and ligands were identified through observations of β 2 ‐integrin dependence in patients with chronic granulomatous disease. Interactions between neutrophils and the apical membrane of crypt cells were analysed using HPLC and an in vitro model with T84 monolayers colonized by Salmonella typhimurium was used to assess neutrophil movement across the epithelium. The decline in transepithelial resistance following movement of neutrophils across the epithelial monolayer may have been due to an interaction between neutrophils and ligand ICAM‐1 in which the neutrophils move along the paracellular pathway of epithelial cells. Cell surface polarity may influence these neutrophil‐epithelial interactions which influence Cl secretion. These studies revealed that only strains produced in vivo were able to induce neutrophil transmigration in the in vitro model and may be indicative of new progressive therapies for inflammatory bowel disease.