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Milk and egg phospholipids act as protective surfactants against luminal acid in Necturus gastric mucosa
Author(s) -
KIVINEN A.,
TARPILA S.,
KIVILUOTOS T.,
MUSTONENS H.,
KIVILAAKSOS E.
Publication year - 1995
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.1995.tb00439.x
Subject(s) - necturus , epithelium , intracellular , gastric mucosa , apical membrane , antrum , paracellular transport , chemistry , pulmonary surfactant , basal (medicine) , taurocholic acid , biochemistry , epithelial polarity , phospholipid , medicine , biophysics , membrane , endocrinology , biology , stomach , pathology , bile acid , insulin , permeability (electromagnetism)
SUMMARY Background : Our previous studies indicate that milk phospholipids have anti‐ulcer properties in rats and humans, possibly by forming a hydrophobic surfactant layer at the epithelial surface. In the present study we measured intracellular pH and parameters of membrane resistances in gastric epithelium exposed to luminal acid using a microelectrode technique. Methods : Chambered isolated Necturus maculosus antral mucosa was exposed to pH 2.3, with or without 20–2 5 min pre‐treatment with milk or egg phospholipids. The pH in surface epithelial cells was measured with double‐barrelled liquid sensor pH/PDmicroelectrodes. Results : Pre‐treatment with phospholipids (2500–5000 μ g P/mL) significantly ( P < 0.01 n = 14) opposed intracellular acidification. Phospholipids significantly ( P < 0.05 n = 14) increased the ratio of apical and basal membrane resistances, suggesting that they primarily affect the apical cell membrane. In contrast, there was no significant change in transmucosal resistance suggesting lack of effect on paracellular shunts in the‘leaky’ epithelium. Conclusions: Exogenous phospholipids of dietary origin may be used to form a protective layer in the gastric mucosa against irritants.