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Effects of PGE 2 , carbenoxolone, cholecystokinin, and the thromboxane‐mimetic u46619 on protein and glycoprotein production by isolated pig gastric mucosal cells
Author(s) -
HEIM H.K.,
OESTMANN A.,
SEWING K.Fr.
Publication year - 1990
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.1990.tb00494.x
Subject(s) - carbenoxolone , endocrinology , thromboxane , medicine , prostaglandin , stimulation , prostaglandin e , mucus , secretion , chemistry , biochemistry , biology , platelet , ecology , intracellular , gap junction
SUMMARY We studied the effects of prostaglandin (PG) E 2 , carbenoxolone, cholecystokinin‐octapeptide (CCK‐OP), and the thromboxane‐mimetic U46619, all known to stimulate gastric mucus secretion in vivo , on protein and glycoprotein synthesis in and release from isolated and enriched pig gastric mucous cells, as measured by the incorporation of [ 3 H]L‐leucine and N‐acetyl‐[ 14 C]D‐glucosamine respectively into cellular and released acid insoluble material. PGE 2 stimulated glycoprotein and protein synthesis (EC 50 7 and 30 nmol/L, respectively) and release (EC 50 50 and 140 nmol/L, respectively) in a concentration‐dependent manner, whereas carbenoxolone, CCK‐OP and U46619 failed to enhance the incorporation of the tracers. We conclude that stimulation of mucus secretion by PGE 2 is related to direct effects on protein and glycoprotein production of gastric non‐parietal cells, whereas indirect effects may be involved in the stimulation by carbenoxolone, CCK‐OP, and U46619.