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Effect of the bile‐acid sequestrant colestipol on postprandial serum bile‐acid concentration: evaluation by bioluminescent enzymic analysis
Author(s) -
ROSSI S. S.,
WAYNE M. L.,
SMITH R. B.,
WRIGHT C. E.,
ANDREADIS N. A.,
HOFMANN A. F.
Publication year - 1989
Publication title -
alimentary pharmacology and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.308
H-Index - 177
eISSN - 1365-2036
pISSN - 0269-2813
DOI - 10.1111/j.1365-2036.1989.tb00189.x
Subject(s) - bile acid , postprandial , medicine , chenodeoxycholic acid , ingestion , deoxycholic acid , endocrinology , chromatography , chemistry , insulin
SUMMARY Chronic ingestion of bile‐acid sequestrants has been shown to decrease the serum cholesterol concentration and coronary events in hypercholesterolaemic patients. To develop improved sequestrants, a rapid, convenient method for testing the bile‐acid binding efficacy of sequestrants is needed. Serum bile‐acid concentrations could be used to detect bile‐acid binding by an administered sequestrant, since the serum bile‐acid concentration is determined largely by the rate of intestinal absorption in healthy individuals. To test this, serum bile‐acid concentrations were measured at frequent intervals over 24 h in five otherwise healthy hypercholesterolaemic subjects during the ingestion of three standard meals, with or without the addition of 5 g colestipol granules administered 30 min before each meal. Total serum bile‐acid concentration was measured with a previously reported bioluminescent enzymic assay, that uses a 3α‐hydroxysteroid dehydrogenase, an oxido‐reductase, and a bacterial luciferase co‐immobilized on to Sepharose beads. Bile acids in 1 ml of serum were isolated by solid‐phase extraction chromatography with reversed‐phase C18 cartridges.