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Mining the gene repertoire and ESTs for G protein‐coupled receptors with evolutionary perspective
Author(s) -
Schiöth H. B.,
Nordström K. J. V.,
Fredriksson R.
Publication year - 2007
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1365-201x.2007.01694.x
Subject(s) - g protein coupled receptor , biology , expressed sequence tag , computational biology , genome , gene , pseudogene , genetics , frizzled , repertoire , dna microarray , receptor , evolutionary biology , wnt signaling pathway , gene expression , physics , acoustics
Abstract The purpose of this article was to review recent progress in mining the gene repertoire and expressed sequence tags (ESTs) for the super‐family of G protein‐coupled receptors (GPCRs) in the form of a proceeding from the Nordic GPCR meeting held at the Nobel Forum, Karolinska Institute in August 2006. We update and give an overview of the expansion of the main families of GPCRs; Glutamate , Rhodopsin , Adhesion , Frizzled and Secretin (GRAFS) in perspective of fully sequenced genomes. We look into the most recent findings including the work that has been carried out on the spotted green puffer fish ( Tetraodon nigroviridis ), mouse ( Mus musculus ), chicken ( Gallus gallus ), slime mold ( Dictyostelium discoideum ) and the plant pathogenic fungus Magnaporthe grisea . We use examples from our recent work on chicken GPCRs to highlight the importance of detailed assembly and curation of sequences and how that can affect percentage similarity and phylogeny. ESTs can give valuable information about expression patterns. GPCRs have comparatively low numbers of EST suggesting that GPCRs are in generally expressed in lower amount than other genes. We discuss similarities in the evolution of the trace amine associated receptors with other sensory receptors.