Open AccessTransposition of the miniature inverted‐repeat transposable element mimp1 in the wheat pathogen F usarium culmorum
Author(s) -
Spanu Francesca,
Pasquali Matias,
Scherm Barbara,
Balmas Virgilio,
Marcello Angela,
Ortu Giuseppe,
Dufresne Marie,
Hoffmann Lucien,
Daboussi MarieJosée,
Migheli Quirico
Publication year - 2012
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2012.00823.x
Subject(s) - fusarium culmorum , biology , transposable element , fusarium oxysporum , transposase , transposition (logic) , genome , genetics , inverted repeat , gene , fusarium , computational biology , linguistics , philosophy
Summary High‐throughput methods are needed for functional genomics analysis in F usarium culmorum , the cause of crown and foot rot on wheat and a type B trichothecene producer. Our aim was to develop and test the efficacy of a double‐component system based on the ability of the impala transposase to transactivate the miniature inverted‐repeat transposable element mimp1 of F usarium oxysporum . We report, for the first time, the application of a tagging system based on a heterologous transposon and of splinkerette‐polymerase chain reaction to identify mimp1 flanking regions in the filamentous fungus F . culmorum . Similar to previous observations in F usarium graminearum , mimp1 transposes in F . culmorum by a cut‐and‐paste mechanism into TA dinucleotides, which are duplicated on insertion. mimp1 was reinserted in open reading frames in 16.4% (i.e. 10 of 61) of the strains analysed, probably spanning throughout the entire genome of F . culmorum . The effectiveness of the mimp1/impala double‐component system for gene tagging in F . culmorum was confirmed phenotypically for a putative aurofusarin gene. This system also allowed the identification of two genes putatively involved in oxidative stress‐coping capabilities in F . culmorum , as well as a sequence specific to this fungus, thus suggesting the valuable exploratory role of this tool.