Open AccessComparison of transcript profiles in different life stages of the nematode Globodera pallida under different host potato genotypes
Author(s) -
PalomaresRius Juan E.,
Hedley Pete E.,
Cock Peter J. A.,
Morris Jenny A.,
Jones John T.,
Vovlas Nikos,
Blok Vivian
Publication year - 2012
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2012.00821.x
Subject(s) - biology , globodera pallida , cultivar , globodera rostochiensis , nematode , host (biology) , gene , inoculation , potato cyst nematode , botany , genotype , horticulture , genetics , solanaceae , ecology
Summary The potato cyst nematodes ( PCN s) G lobodera pallida and G lobodera rostochiensis are important parasites of potato. PCN s undergo complex biotrophic interactions with their hosts that involve gene expression changes in both the nematode and the host plant. The aim of this study was to determine key genes that are differentially expressed in G lobodera pallida life cycle stages and during the initiation of the feeding site in susceptible and partially resistant potato genotypes. For this purpose, two microarray experiments were designed: (i) a comparison of eggs, infective second‐stage juveniles ( J 2s) and sedentary parasitic‐stage J 2s ( SJ 2); (ii) a comparison of SJ 2s at 8 days after inoculation ( DAI ) in the susceptible cultivar ( D esirée) and two partially resistant lines. The results showed differential expression of G . pallida genes during the stages studied, including previously characterized effectors. In addition, a large number of genes changed their expression between SJ 2s in the susceptible cultivar and those infecting partially resistant lines; the number of genes with modified expression was lower when the two partially resistant lines were compared. Moreover, a histopathological study was performed at several time points (7, 14 and 30 DAI ) and showed the similarities between both partially resistant lines with a delay and degeneration in the formation of the syncytia in comparison with the susceptible cultivar. Females at 30 DAI in partially resistant lines showed a delay in their development in comparison with those in the susceptible cultivar.