Open AccessSnTox5 – Snn5 : a novel S tagonospora nodorum effector–wheat gene interaction and its relationship with the SnToxA – Tsn1 and SnTox3 – Snn3 – B1 interactions
Author(s) -
Friesen Timothy l.,
Chu Chenggen,
Xu Steven S.,
Faris Justin D.
Publication year - 2012
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2012.00819.x
Subject(s) - effector , biology , population , gene , protein–protein interaction , pathogen , genetics , microbiology and biotechnology , demography , sociology
Summary The S tagonospora nodorum –wheat interaction involves multiple pathogen‐produced necrotrophic effectors that interact directly or indirectly with specific host gene products to induce the disease S tagonospora nodorum blotch ( SNB ). Here, we used a tetraploid wheat mapping population to identify and characterize a sixth effector–host gene interaction in the wheat– S . nodorum system. Initial characterization of the effector SnTox5 indicated that it is a proteinaceous necrotrophic effector that induces necrosis on host lines harbouring the Snn5 sensitivity gene, which was mapped to the long arm of wheat chromosome 4B . On the basis of ultrafiltration, SnTox5 is probably in the size range 10–30 kDa . Analysis of SNB development in the mapping population indicated that the SnTox5 – Snn5 interaction explains 37%–63% of the variation, demonstrating that this interaction plays a significant role in disease development. When the SnTox5 – Snn5 and SnToxA – Tsn1 interactions occurred together, the level of SNB was increased significantly. Similar to several other interactions in this system, the SnTox5 – Snn5 interaction is light dependent, suggesting that multiple interactions may exploit the same pathways to cause disease.