Open AccessPossible involvement of maize Rop1 in the defence responses of plants to viral infection
Author(s) -
CAO YANYONG,
SHI YAN,
LI YONGQIANG,
CHENG YUQIN,
ZHOU TAO,
FAN ZAIFENG
Publication year - 2012
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2011.00782.x
Subject(s) - biology , nicotiana benthamiana , potyvirus , gene , virology , virus , gene silencing , suppression subtractive hybridization , heterologous , complementary dna , gene expression , plant virus , potato virus x , cdna library , genetics
SUMMARY The expression of host genes can be altered during the process of viral infection. To investigate the viral infection‐induced up‐regulated gene expression changes of maize at different time intervals post‐inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was constructed. A total of 454 cDNA clones were identified to be viral infection‐induced up‐regulated genes. The influence of Rop1 on the infection of maize by SCMV was investigated. The results showed that transient silencing of the ZmRop1 gene through virus‐induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus ( Pennisetum mosaic virus ) in maize plants, whereas transient over‐expression of ZmRop1 in maize protoplasts reduced SCMV accumulation. Furthermore, it was demonstrated that the heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants. These data suggest that ZmRop1 may play a role in plant defence responses to viral infection.