Open AccessAnalysis of polymorphism and transcription of the effector gene Avr1b in Phytophthora sojae isolates from China virulent to Rps1b
Author(s) -
CUI LINKAI,
YIN WEIXIAO,
DONG SUOMENG,
WANG YUANCHAO
Publication year - 2012
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2011.00733.x
Subject(s) - phytophthora sojae , biology , virulence , effector , genetics , gene , china , polymorphism (computer science) , phytophthora , genotype , botany , microbiology and biotechnology , political science , law
SUMMARY The effector gene Avr1b‐ 1 of Phytophthora sojae determines the efficacy of the resistance gene Rps1b in soybean. The sequences of the Avr1b‐ 1 locus in 34 Chinese isolates of P. sojae were obtained and analysed by polymerase chain reaction (PCR) and inverse PCR. Four different alleles and a complete deletion mutation of the Avr1b ‐1 gene were identified. Molecular analysis of the deletion breakpoints in the Avr1b‐ 1 locus revealed that an 8‐kb DNA sequence containing Avr1b‐ 1 was deleted and a 12.7‐kb DNA sequence was inserted at the same locus. A truncated transposase gene was found and five transposable elements were predicted in the inserted sequence, suggesting that the deletion of Avr1b‐ 1 might be attributed to transposon movement. The transcription of Avr1b‐ 1 was analysed in virulent isolates containing four alleles of Avr1b‐ 1 by real‐time reverse transcription‐PCR. In all virulent isolates, only those isolates containing the second allele transcripted Avr1b‐ 1.