Open AccessCellular and transcriptional responses of wheat during compatible and incompatible race‐specific interactions with Puccinia striiformis f. sp. tritici
Author(s) -
BOZKURT TOLGA O.,
MCGRANN GRAHAM R.D.,
MACCORMACK RUTH,
BOYD LESLEY A.,
AKKAYA MAHINUR S.
Publication year - 2010
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2010.00633.x
Subject(s) - biology , transcriptome , gene , hypersensitive response , programmed cell death , rust (programming language) , basidiomycota , microarray analysis techniques , microarray , genetics , gene expression , microbiology and biotechnology , plant disease resistance , botany , apoptosis , computer science , programming language
SUMMARY The initial stages of Puccinia striiformis f. sp. tritici (the causal agent of yellow rust in wheat) infection triggered a hypersensitive cell death (HCD) response in both compatible and Yr1 ‐mediated incompatible interactions, although the response was earlier and more extensive in the incompatible interaction. Later stages of fungal development were only associated with an HCD response in the incompatible interaction, the HCD response being effectively suppressed in the compatible interaction. Cell autofluorescence was seen in mesophyll cells in direct contact with fungal infection hyphae (primary HCD) and in adjacent mesophyll cells (secondary HCD), indicating the activation of cell‐to‐cell signalling. Microarray analysis identified a number of defence‐related transcripts implicated in Yr1 ‐mediated resistance, including classical pathogenesis‐related ( PR ) transcripts and genes involved in plant cell defence responses, such as the oxidative burst and cell wall fortification. A quantitative reverse transcriptase‐polymerase chain reaction time course analysis identified a number of defence‐related genes, including PR2 , PR4 , PR9 , PR10 and WIR1 transcripts, associated with the latter stages of Yr1 ‐mediated resistance. A meta‐analysis comparison of the Yr1 ‐regulated transcriptome with the resistance transcriptomes of the race‐specific resistance gene Yr5 and the race‐nonspecific adult plant resistance gene Yr39 indicated limited transcript commonality. Common transcripts were largely confined to classic PR and defence‐related genes.