Open AccessInvolvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E‐mediated resistance against Lettuce mosaic potyvirus
Author(s) -
ABDULRAZZAK ANAS,
GUIRAUD THOMAS,
PEYPELUT MARTINE,
WALTER JOCELYNE,
HOUVENAGHEL MARIECHRISTINE,
CANDRESSE THIERRY,
LE GALL OLIVIER,
GERMANRETANA SYLVIE
Publication year - 2009
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2008.00513.x
Subject(s) - potyvirus , biology , genetics , eif4e , potyviridae , gene , virology , mutagenesis , virus , plant virus , virulence , mutation , allele , tobacco etch virus , translation (biology) , messenger rna
SUMMARY The capacity of Lettuce mosaic virus to overcome the lettuce resistance conferred by the mo1 1 and mo1 2 alleles of the gene for eukaryotic translation initiation factor 4E (eIF4E) was analysed using reverse genetics. Mutations in the virus genome‐linked protein (VPg) allowed mo1 1 only to be overcome, but mutations in the C‐terminal portion of the cylindrical inclusion (CI) protein allowed both alleles to be overcome. Site‐directed mutagenesis pinpointed a key role of the amino acid at position 621 in the virulence. This is the first example of the involvement of a potyviral CI protein in the breaking of an eIF4E‐mediated resistance.