Open AccessEffect of potato suberin on Streptomyces scabies proteome
Author(s) -
LAUZIER ANNIE,
SIMAOBEAUNOIR ANNEMARIE,
BOURASSA SYLVIE,
POIRIER GUY G.,
TALBOT BRIAN,
BEAULIEU CAROLE
Publication year - 2008
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2008.00493.x
Subject(s) - suberin , biology , secondary metabolism , biochemistry , proteome , streptomyces , enzyme , bacteria , cell wall , biosynthesis , genetics
SUMMARY Two‐dimensional (2D) PAGE was used to detect proteins induced in Streptomyces scabies by potato suberin, a lipidic plant polymer. Nineteen up‐regulated proteins were excised from 2D gels and analysed by N‐terminal sequencing or tandem mass spectrometry (MS/MS). Four of the up‐regulated proteins could be linked to the bacterial response to stress (AldH, GroES, TerD and LexA). Specific metabolic pathways seemed to be activated in the presence of suberin, as shown by the increased expression of specific transporters and of enzymes related not only to glycolysis, but also to nucleotide and amino acid metabolism. Suberin also appeared to influence secondary metabolism as it also caused the overproduction of the BldK proteins that are known to be involved in differentiation and secondary metabolism.