Open AccessAdult plant resistance‐related gene expression in ‘Camp Remy’ wheat inoculated with Puccinia striiformis
Author(s) -
MALLARD STÉPHANIE,
NÈGRE SYLVIE,
POUYA SHALA,
GAUDET DENIS,
LU ZHENXIANG,
DEDRYVER FRANÇOISE
Publication year - 2008
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2007.00459.x
Subject(s) - biology , gene , seedling , genetics , gene expression , inoculation , complementary dna , botany , horticulture
SUMMARY The French wheat variety ‘Camp Remy’ (CR) possesses a durable, adult plant resistance to yellow rust (YR), caused by the pathogen Puccinia striiformis . Using cDNA–AFLP on different sets of heterogeneous inbred families (HIFs) derived from the cross CR × Récital, we compared gene expression profiles during one seedling and two adult developmental stages following inoculation with P. striiformis . Transcripts differentially expressed in response to YR infection were isolated and cloned. Sequence analysis of the resultant clones revealed several classes of putative genes, including those related to resistance/defence responses, transcription and signal transduction, and primary metabolism. The expression profiles of seven selected genes were obtained using real‐time PCR in CR leaves at the same three stages of development. The results confirmed the stage‐specific expression of the genes at one or two specific stages in response to P. striiformis infection and demonstrated that CR modifies the expression of some resistance/defence‐related genes during its transition from the seedling to adult growth stages. These results provided the first clue to understand the molecular basis of quantitative trait loci for adult plant resistance to YR and connect it with durability.