Open AccessTransgenic Arabidopsis plants expressing Agrobacterium tumefaciens VirD2 protein are less susceptible to Agrobacterium transformation
Author(s) -
HWANG HAUHSUAN,
MYSORE KIRANKUMAR S.,
GELVIN STANTON B.
Publication year - 2006
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2006.00353.x
Subject(s) - agrobacterium tumefaciens , agrobacterium , biology , arabidopsis , transformation (genetics) , genetically modified crops , transgene , gall , botany , gene , genetics , mutant
SUMMARY Agrobacterium tumefaciens causes crown gall disease on many plant species and can result in considerable economic losses. Here we report a new strategy to control crown gall disease by over‐expressing Agrobacterium tumefaciens VirD2 protein in plants. Transgenic Arabidopsis plants over‐expressing virD2 from constitutive or wound‐inducible promoters are less susceptible to Agrobacterium ‐mediated transformation. Additionally, the transient introduction of an A. tumefaciens virD2 gene in tobacco BY‐2 cells reduces subsequent Agrobacterium ‐mediated transformation.