Open AccessMolecular characterization of mlo mutants in North American two‐ and six‐rowed malting barley cultivars
Author(s) -
PANSTRUGA RALPH,
MOLINACANO JOSÉ LUIS,
REINSTÄDLER ANJA,
MÜLLER JUDITH
Publication year - 2005
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2005.00277.x
Subject(s) - biology , mutant , gene , hordeum vulgare , allele , open reading frame , genetics , cultivar , genotype , genomic dna , arabidopsis , stop codon , wild type , microbiology and biotechnology , botany , poaceae , peptide sequence
SUMMARY Barley lines PRU1, URS1 and URS2 represent three candidate mlo mutants induced in either the two‐rowed cultivar Prudentia or the six‐rowed cultivar Ursula. Both Prudentia and Ursula are North American malting barley varieties with specific malting properties. Here, we analysed the three candidate mutants at the molecular level. We identified lesions in the Mlo gene of all three lines, causing either a premature stop codon (PRU1), a shift in the reading frame (URS1) or a single amino acid replacement (URS2). In a transient gene expression assay, the URS2 mlo allele fails to complement a barley null mutant genotype, indicating that URS2 is a genuine mlo mutant (here designated as mlo ‐33). The MLO‐33 mutant variant accumulates to similar levels as the wild‐type MLO protein in Arabidopsis protoplasts, suggesting that MLO‐33 is stable in planta . We show that the mlo ‐33 allele can be readily detected in barley genomic DNA by a cleaved amplified polymorphic sequence marker, rendering this allele particularly suited for marker‐assisted breeding.