Open AccessA parasitism gene from a plant‐parasitic nematode with function similar to CLAVATA3/ESR (CLE) of Arabidopsis thaliana
Author(s) -
WANG XIAOHONG,
MITCHUM MELISSA G.,
GAO BINGLI,
LI CHUNYING,
DIAB HANANE,
BAUM THOMAS J.,
HUSSEY RICHARD S.,
DAVIS ERIC L.
Publication year - 2005
Publication title -
molecular plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.945
H-Index - 103
eISSN - 1364-3703
pISSN - 1464-6722
DOI - 10.1111/j.1364-3703.2005.00270.x
Subject(s) - arabidopsis , biology , meristem , arabidopsis thaliana , mutant , phenotype , microbiology and biotechnology , gene , transgene , nematode , nematode infection , botany , genetics , ecology
SUMMARY The Hg‐SYV46 parasitism gene is expressed exclusively in the dorsal oesophageal gland cell of parasitic stages of the soybean cyst nematode, Heterodera glycines , and it encodes a secretory protein that contains a C‐terminal motif of the CLAVATA3/ESR‐related (CLE) family in Arabidopsis thaliana . In shoot and floral meristems of Arabidopsis, the stem cells secret CLV3, a founding member of the CLE protein family, that activates the CLV1/CLV2 receptor complex and negatively regulates WUSCHEL expression to restrict the size of the stem cell population. Mis‐expression of Hg‐SYV46 in Arabidopsis (ecotype Columbia‐0) under control of the CaMV35S promoter resulted in a wus ‐like phenotype including premature termination of the shoot apical meristem and the development of flowers lacking the central gynoecium. The wus ‐like phenotype observed was similar to reports of over‐expression of CLV3 and CLE40 in Arabidopsis, as was down‐regulation of WUS expression in the shoot apices of 35S::Hg‐SYV46/Col‐0 plants. Expression of 35S::Hg‐SYV46 in a clv3‐ 1 mutant of Arabidopsis was able partially or fully to rescue the mutant phenotype, probably dependent upon localization and level of transgene expression. A short root phenotype, as reported for over‐expression of CLV3 , CLE40 and CLE19 in roots, was also produced in primary 35S::Hg‐SYV46/Col‐0 transgenic plants. The results suggest a functional similarity of HG‐SYV46 to plant‐secreted CLE ligands that may play a role in the differentiation or division of feeding cells induced in plant roots by parasitic nematodes.