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Pmr1, a P‐type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast: The importance of Mn 2+ homeostasis
Author(s) -
Maeda Takuya,
Sugiura Reiko,
Kita Ayako,
Saito Mariko,
Deng Lu,
He Yi,
Yabin Lu,
Fujita Yasuko,
Takegawa Kaoru,
Shuntoh Hisato,
Kuno Takayoshi
Publication year - 2004
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1111/j.1356-9597.2004.00699.x
Subject(s) - biology , schizosaccharomyces pombe , microbiology and biotechnology , mutant , saccharomyces cerevisiae , homeostasis , egta , morphogenesis , cell , atpase , phenotype , gene , schizosaccharomyces , yeast , biochemistry , calcium , chemistry , enzyme , organic chemistry
Schizosaccharomyces pombe pmr1+ gene is homologous to Saccharomyces cerevisiae PMR1 gene, which encodes the P‐type Ca 2+ /Mn 2+ ‐ATPase. Addition of Mn 2+ , as well as Ca 2+ , to the medium induced pmr1 + gene expression in a calcineurin‐dependent manner. The pmr1 knockout ( Δ pmr1 ) cells exhibited hypersensitivity to EGTA. A screen for high gene dosage‐suppressors of the EGTA‐hypersensitive phenotype of Δ pmr1 led to the identification of pdt1 + gene, which encodes an Nramp ‐related metal transporter. The Δ pmr1 cells showed round cell morphology. Although Δ pdt1 cells appeared normal in the regular medium, it showed round cell morphology similar to that of the Δ pmr1 cells when Mn 2+ was removed from the medium. The removal of Mn 2+ also exacerbated the round morphology of the Δ pmr1 cells. The Δ pmr1 Δ pdt1 double mutants grew very slowly and showed extremely aberrant cell morphology with round, enlarged and depolarized shape. The addition of Mn 2+ , but not Ca 2+ , to the medium completely suppressed the morphological defects, while both Mn 2+ and Ca 2+ markedly improved the slow growth of the double mutants. These results suggest that Pmr1 and Pdt1 cooperatively regulate cell morphogenesis through the control of Mn 2+ homeostasis, and that calcineurin functions as a Mn 2+ sensor as well as a Mn 2+ homeostasis regulator.