Open AccessProfiling oxidative DNA damage: Effects of antioxidants
Author(s) -
Box Harold C.,
Patrzyc Helen B.,
Budzinski Edwin E.,
Dawidzik Jean B.,
Freund Harold G.,
Zeitouni Nathalie C.,
Mahoney Martin C.
Publication year - 2012
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2012.02391.x
Subject(s) - antioxidant , oxidative stress , dna damage , tandem mass spectrometry , chemistry , dna , biochemistry , medicine , pharmacology , chromatography , mass spectrometry
The goal of this research was to determine whether antioxidant usage could be correlated with changes in DNA damage levels. Liquid C hromatography‐tandem M ass S pectrometry ( LC ‐ MS / MS ) was used to simultaneously measure five different oxidatively‐induced base modifications in the DNA of WBC . Measurements of the five modifications were made before and after an 8‐week trial during which participants took the SU . VI . MAX supplement. Levels of the five DNA modifications were compared among different groupings: users versus non‐users of antioxidant supplements, before versus after the supplement intervention and men versus women. The statistical significance of differences between groups was most significant for pyrimidine base modifications and the observed trends reflect trends reported in epidemiological studies of antioxidant usage. A combination of modifications derived from pyrimidine bases is suggested as a superior indicator of oxidative stress.