Open AccessGirdin locates in centrosome and midbody and plays an important role in cell division
Author(s) -
Mao JingZhuo,
Jiang Ping,
Cui SuPing,
Ren YaLi,
Zhao Jing,
Yin XiuHua,
Enomoto Atsushi,
Liu HaiJing,
Hou Lin,
Takahashi Masahide,
Zhang Bo
Publication year - 2012
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2012.02378.x
Subject(s) - midbody , microbiology and biotechnology , centrosome , cell growth , biology , mitosis , cell division , cytokinesis , cell cycle , cell , genetics
Girdin is a downstream effector of epidermal growth factor receptor ( EGFR )‐ AKT and interacts with actin and microtubule. Increasing evidence confirmed that G irdin played an important role in cell migration. Here we report that G irdin also regulates cell division. Overexpression or suppression of G irdin leads to attenuated cell proliferation. Imaging of mitotic cells revealed that G irdin is located in the cell division apparatus such as centrosome and midbody. The sub‐cellular localization of G irdin was dependent on the domains, which interacted with actin or microtubules. Overexpression of G irdin lead to increased centrosome splitting and amplification. In addition, data show that p AKT also locates in both the centrosome and midbody, indicating the regulating role of AKT in G irdin‐mediated cell division. To elucidate the effect of G irdin on tumor growth in vivo , H e L a cells infected with retrovirus harboring either control or G irdin sh RNA s were injected subcutaneously into the immunocompromised nude mice. Downregulation of G irdin by sh RNA markedly inhibited the cell growth of subcutaneously transplanted tumors in nude mice. These data demonstrate that G irdin is important for efficient cell division. Taking our previous data into consideration, we speculate that G irdin regulates both cell division and cell migration through cytoskeletal molecules.