Retracted: Antitumor activity of human γδ T cells transducted with CD 8 and with T ‐cell receptors of tumor‐specific cytotoxic T lymphocytes

The difficulty in the induction and preparation of a large number of autologous tumor‐specific cytotoxic T lymphocytes ( CTL ) from individual patients is one of major problems in their application to adoptive immunotherapy. The present study tried to establish the useful antitumor effectors by using γδ T cells through tumor‐specific TCR αβ genes transduction, and evaluated the efficacy of their adoptive transfer in a non‐obese diabetic/severe combined immunodeficiency (NOD/SCID) mice model. The TCR αβ gene was cloned from the HLA ‐ B 15‐restricted CTL clone specific of the K ita‐ K yushu L ung C ancer antigen‐1 ( KK ‐ LC ‐1). The cloned TCR αβ as well as the CD 8 gene were transduced into γδ T cells induced from peripheral blood lymphocytes ( PBL ). Cytotoxic T lymphocyte activity was examined using a standard 4 h 51 C r release assay. Mice with a xenotransplanted tumor were treated with an injection of effector cells. Successful transduction of TCR αβ was confirmed by the staining of KK ‐ LC ‐1‐specific tetramers. The γδ T cells transduced with TCR αβ and CD 8 showed CTL activity against the KK ‐ LC ‐1‐positive lung cancer cell line in a HLA B 15‐restricted manner. Adoptive transfer of the effector cells in a mice model resulted in marked growth suppression of KK ‐ LC ‐1‐ and HLA ‐ B 15‐positive xenotransplanted tumors. Co‐transducing TCR αβ and CD 8 into γδ T cells yielded the same antigen‐specific activity as an original CTL in vitro and in vivo . The TCR αβ gene transduction into γδ T cells is a promising strategy for developing new adoptive immunotherapy. ( Cancer Sci , doi: 10.1111/j.1349‐7006.2012.02337.x, 2012)