Open AccessAnti‐angiogenesis effect of 3′‐sulfoquinovosyl‐1′‐monoacylglycerol via upregulation of thrombospondin 1
Author(s) -
Matsuki Kayo,
Tanabe Atsushi,
Hongo Ayumi,
Sugawara Fumio,
Sakaguchi Kengo,
Takahashi Nobuaki,
Sato Noriyuki,
Sahara Hiroeki
Publication year - 2012
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2012.02333.x
Subject(s) - downregulation and upregulation , thrombospondin 1 , angiogenesis , gene knockdown , in vivo , chemistry , microbiology and biotechnology , cancer research , biology , biochemistry , gene , genetics
We previously reported that 3′‐sulfoquinovosyl‐1′‐monoacylglycerol ( SQMG ) effectively suppresses the growth of solid tumors, likely via its anti‐angiogenic activity. To investigate how SQMG affects angiogenesis, we performed DNA microarray analysis and quantitative real‐time polymerase chain reaction. Consequently, upregulation of thrombospondin 1 ( TSP ‐1) in SQMG ‐treated tumors in vitro and in vivo was confirmed. To address the mechanisms of TSP ‐1 upregulation by SQMG , we established stable TSP ‐1‐knockdown transformants ( TSP 1‐ KT ) by short hairpin RNA induction and performed reporter assay and in vivo assessment of anti‐tumor assay. On the reporter assay, transcriptional upregulation of TSP ‐1 in TSP 1‐ KT could not be induced by SQMG , thus suggesting that TSP ‐1 upregulation by SQMG occurred via TSP ‐1 molecule. In addition, growth of TSP 1‐ KT xenografted tumors in vivo was not inhibited by SQMG , thus suggesting that anti‐angiogenesis via TSP ‐1 upregulation induced by SQMG did not occur, as the SQMG target molecule TSP ‐1 was knocked down in TSP 1‐ KT transformants. These data provide that SQMG is a promising candidate for the treatment of tumor‐induced angiogenesis via TSP ‐1 upregulation. ( Cancer Sci, doi: 10.1111/j.1349‐7006.2012.02333.x, 2012)