Open AccessOverexpression of ADP ‐ribosylation factor 1 in human gastric carcinoma and its clinicopathological significance
Author(s) -
Tsai MingMing,
Lin Paul Y.,
Cheng WanLi,
Tsai ChungYing,
Chi HsiangCheng,
Chen ChengYi,
Tseng YiHsin,
Cheng YiFen,
Chen ChiDe,
Liang Ying,
Liao ChiaJung,
Wu ShengMing,
Lin YangHsiang,
Chung IHsiao,
Wang ChiaSiu,
Lin KwangHuei
Publication year - 2012
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2012.02243.x
Subject(s) - immunohistochemistry , lymphovascular invasion , cancer , western blot , metastasis , pathology , biomarker , cancer research , medicine , biology , gene , biochemistry
Gastric cancer is the sixth leading cause of cancer‐related death in T aiwan, and the identification of related factors is essential to increase patient survival. ADP ‐ribosylation factor 1 ( ARF 1) was initially identified using 2‐ D electrophoresis combined with MALDI –time‐of‐flight mass spectrometry. ADP ‐ribosylation factor 1 belongs to the R as superfamily or GTP ‐binding protein family and has been shown to enhance cell proliferation. In the current study, we evaluated the potential of ARF 1 as a biomarker for gastric cancer detection. ADP ‐ribosylation factor 1 m RNA was upregulated in tumor tissues (compared with adjacent non‐tumor tissues, n = 55) in approximately 67.2% of gastric cancer patients. Expression of ARF 1 protein was additionally observed using W estern blot and immunohistochemistry ( IHC ) analyses. The clinicopathological correlations of ARF 1 were further evaluated. Elevated ARF 1 expression was strongly correlated with lymph node metastasis ( P = 0.008), serosal invasion ( P = 0.046), lymphatic invasion ( P = 0.035), and pathological staging ( P = 0.010). Moreover, the 5‐year survival rate for the lower ARF 1 expression group ( n = 50; IHC score < 90) was higher than that of the higher expression group ( n = 60; IHC score ≥ 90) ( P = 0.0228, log–rank test). To establish the specific function of ARF 1 in human gastric cancer, isogenic ARF1 ‐overexpressing cell lines were prepared. Our results showed that ARF 1 ‐overexpressing clones display enhanced cell proliferation, migration, and invasion. Furthermore, ARF 1 ‐overexpression might contribute to poor prognosis of patients. These findings collectively support the utility of ARF 1 as a novel prognostic marker for gastric cancer and its role in cell invasion. Cancer Sci 2012; 103: 1136–1144)