Open AccessRole of activation‐induced cytidine deaminase in the progression of follicular lymphoma
Author(s) -
Shikata Hisaharu,
Yakushijin Yoshihiro,
Matsushita Natsuki,
Sakai Akira,
Sugita Atsuro,
Nakamura Naoya,
Yamanouchi Jun,
Azuma Taichi,
Hato Takaaki,
Yasukawa Masaki
Publication year - 2012
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2011.02186.x
Subject(s) - activation induced (cytidine) deaminase , somatic hypermutation , cytidine deaminase , germinal center , follicular lymphoma , immunoglobulin class switching , biology , cancer research , lymphoma , microbiology and biotechnology , transfection , antibody , b cell , cell culture , andrology , immunology , medicine , genetics
Activation‐induced cytidine deaminase (AID/AICDA) is required for somatic hypermutation and class‐switch recombination of the immunoglobulin gene, and for c‐myc translocation of germinal center‐derived B‐cell lymphoma. In the present study, we attempted to clarify the significance of AID associated with c‐myc in the progression of follicular lymphoma (FL) using RT‐PCR and quantitative real‐time PCR. Tissues from the patients with grade 3 FL expressed relatively higher levels of c‐myc and AID . The samples taken from a patient with FL who died within 2 years after the start of treatment showed either no or low expression of AID , despite expressing high levels of c‐myc . In order to examine the role of AID expression in rapidly progressive FL, the full‐length AID transcript was transfected into AID ‐negative cell lines established from different patients with rapidly progressive FL. This led to the establishment of AID ‐expressing transfectants with a low proliferation rate and a significantly increased incidence of G 0 /G 1 arrest compared with controls. Our results indicate that AID may act as a negative regulator of cell survival in FL when sufficient c‐myc is expressed. Switch‐off or low expression of AID after c‐myc amplification may correlate with the clinical outcomes of FL. ( Cancer Sci 2012; 103: 415–421)