Open AccessPotential anti‐cancer activity of N‐hydroxy‐7‐(2‐naphthylthio) heptanomide (HNHA), a histone deacetylase inhibitor, against breast cancer both in vitro and in vivo
Author(s) -
Park Ki Cheong,
Kim Seung Won,
Park Ji Hyun,
Song Eun Hye,
Yang Jeong Won,
Chung Hyun Joo,
Jung Hye Jin,
Suh Jin Suck,
Kwon Ho Jeong,
Choi Seung Hoon
Publication year - 2011
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2010.01798.x
Subject(s) - cancer , in vivo , histone deacetylase , angiogenesis , cancer research , histone deacetylase inhibitor , cancer cell , breast cancer , vorinostat , biology , in vitro , pharmacology , neovascularization , chemistry , medicine , histone , biochemistry , microbiology and biotechnology , gene
Histone deacetylase (HDAC) is an attractive target for cancer therapy because it plays a key role in gene expression and carcinogenesis. N‐hydroxy‐7‐(2‐naphthylthio) heptanomide (HNHA) is a novel synthetic HDAC inhibitor (HDACI) that shows better pharmacological properties than a known HDACI present in the human fibrosarcoma cell: suberoylanilide hydroxamic acid (SAHA). Here, we investigate the anti‐cancer activity of HNHA against breast cancer both in vitro and in vivo . HNHA arrested the cell cycle at the G 1 /S phase via p21 induction, which led to profound inhibition of cancer cell growth in vitro . In addition, HNHA‐treated cells showed markedly decreased levels of VEGF and HIF‐1α than SAHA and fumagillin (FUMA) when accompanied by increased histone acetylation. HNHA significantly inhibited tumor growth in an in vivo mouse xenograft model. HNHA‐treated mice survived significantly longer than SAHA‐ and FUMA‐treated mice. Dynamic MRI showed significantly decreased blood flow in the HNHA‐treated mice, implying that HNHA inhibits tumor neovascularization. This finding was accompanied by marked reductions of proangiogenic factors and significant induction of angiogenesis inhibitors in tumor tissues. We have shown that HNHA is an effective anti‐tumor agent in breast cancer cells in vitro and in breast cancer xenografts in vivo . Collectively, these findings indicate that HNHA may be a potent anti‐cancer agent against breast cancer due to its multi‐faceted inhibition of HDAC activity, as well as anti‐angiogenesis activity. ( Cancer Sci 2011; 102: 343–350)