Open AccessOsteopontin is a new target molecule for ovarian clear cell carcinoma therapy
Author(s) -
Matsuura Motoki,
Suzuki Takahiro,
Saito Tsuyoshi
Publication year - 2010
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2010.01615.x
Subject(s) - osteopontin , integrin , ovarian carcinoma , extracellular matrix , cancer research , biology , cell adhesion molecule , cell , ovarian cancer , clear cell carcinoma , immunofluorescence , cell culture , carcinoma , pathology , cancer , medicine , microbiology and biotechnology , endocrinology , immunology , antibody , genetics
Recent studies have demonstrated overexpression of osteopontin (OPN) in ovarian clear cell carcinoma. Here, we revealed the role of OPN in invasiveness in ovarian clear cell carcinoma. We used immunofluorescence analysis to detect OPN in a total of 160 patient‐derived specimens. Ovarian clear cell carcinoma cell lines, RMG‐1 and TOV‐21G, were used to monitor changes in OPN and integrin levels, and cell invasiveness following treatment with OPN, simvastatin, and transfection with siRNA. Immunofluorescence analysis revealed statistically significant differences among the histological groups, and ovarian clear cell carcinoma expressed a strong OPN signal. The OPN receptors, alpha v and 5, and beta 1 and 3 integrins, were increased after treatment with OPN. Invasion assays indicated that OPN enhanced in vitro extracellular matrix invasion dose‐dependently in ovarian clear cell carcinoma. Simvastatin significantly reduced expression of OPN and the integrins, and decreased ECM invasion. RNA interference also suppressed ECM invasion. These results suggest that down‐ or up‐regulation of OPN is involved in carcinoma cell invasion. We thus conclude that OPN regulation could have a crucial role in ovarian clear cell carcinoma therapy. ( Cancer Sci 2010)