Open AccessNovel chemoembolization using calcium‐phosphate ceramic microsphere incorporating TNP‐470, an anti‐angiogenic agent
Author(s) -
Emoto Makoto,
Naganuma Yasuko,
Choijamts Batsuren,
Ohno Toshiki,
Yoshihisa Hajime,
Kanomata Nobuhiko,
Kawarabayashi Tatsuhiko,
Aizawa Mamoru
Publication year - 2010
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2009.01479.x
Subject(s) - microsphere , vascularity , in vivo , angiogenesis , chemotherapy , sarcoma , medicine , calcium , cancer research , pathology , biology , microbiology and biotechnology , chemical engineering , engineering
The purpose of the present study was to develop a new method of chemoembolization to improve the therapeutic effectiveness and safety profile of cancer treatment. A chemoembolization approach was designed for human solid tumors using resorbable calcium‐phosphate ceramic microspheres loaded with an agent anti‐angiogenic to tumor vasculature in vivo . The human uterine sarcoma cell line FU‐MMT‐3 was used in this study because this tumor is aggressive and also exhibits a poor response to radiotherapy or any chemotherapy currently used. The calcium‐phosphate ceramic microspheres loaded with TNP‐470, an anti‐angiogenic agent, were injected into FU‐MMT‐3 xenografts in nude mice three times per week for 8 weeks. The treatment using TNP‐470‐loaded microspheres suppressed tumor growth, compared to treatment with TNP‐470 alone, microspheres alone, and the control. The mean tumor weight after treatment using TNP‐470‐loaded microspheres was significantly lower than that after treatment with microspheres alone. These ceramic microspheres were remarkably embolized in tumor microvessels as well as in the feeding arteries and a significant reduction of intratumoral vascularity was also demonstrated following treatment with TNP‐470‐loaded microspheres. Severe loss of body weight was not observed in any mice treated with the TNP‐470‐loaded microspheres, compared to treatment with TNP‐470 alone. These results suggest that targeting tumor vasculature in human uterine sarcoma using calcium‐phosphate microspheres might be more effective and safer than the treatment that employs anti‐angiogenic agent alone. This new chemoembolization method incorporating an anti‐angiogenic agent may contribute to the effective treatment of locally advanced or recurrent solid tumors. ( Cancer Sci 2010; 101: 984–990)