Open AccessViral shedding after p53 adenoviral gene therapy in 10 cases of esophageal cancer
Author(s) -
Kawahira Hiroshi,
Matsushita Kazuyuki,
Shiratori Tooru,
Shimizu Takanori,
Nabeya Yoshihiro,
Hayashi Hideki,
Ochiai Takenori,
Matsubara Hisahiro,
Shimada Hideaki
Publication year - 2010
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2009.01381.x
Subject(s) - saliva , feces , urine , dna , virology , biology , viral vector , esophageal cancer , cancer , gene , microbiology and biotechnology , genetics , biochemistry , recombinant dna
We detected adenoviral DNA fragments in excretions of 10 esophageal cancer patients by DNA‐PCR after tumor injection of Ad‐CMV‐vector. A total of 220 samples consisting of feces, gargling saliva, urine, and blood plasma were assessed. A total of 29.7% of feces samples and 13.2% of gargling saliva samples were positive for adenoviral DNA fragments, but 89.7% of the positive feces samples and all of the positive gargling saliva samples turned negative on day 12 after tumor injection. Although adenoviral DNA fragments may be pathogen‐free, patients’ feces and gargling saliva contain adenoviral DNA fragments for 12 days after injection. ( Cancer Sci 2010; 101: 289–291)