Open AccessInvolvement of G‐patch domain containing 2 overexpression in breast carcinogenesis
Author(s) -
Lin MengLay,
Fukukawa Chikako,
Park JaeHyun,
Naito Kie,
Kijima Kyoko,
Shimo Arata,
Ajiro Masahiko,
Nishidate Toshihiko,
Nakamura Yusuke,
Katagiri Toyomasa
Publication year - 2009
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2009.01185.x
Subject(s) - breast cancer , carcinogenesis , western blot , cancer research , cancer , biology , cancer cell , gene expression , cell growth , gene , genetics
Through analysis of the detailed genome‐wide gene expression profiles of 81 breast tumors, we identified a novel gene, G‐patch domain containing 2 ( GPATCH2 ), that was overexpressed in the great majority of breast cancer cases. Treatment of breast cancer cells MCF‐7 and T47D with siRNA against GPATCH2 effectively suppressed its expression, and resulted in the growth suppression of cancer cells, suggesting its essential role in breast cancer cell growth. We found an interaction of GPATCH2 protein with hPrp43, an RNA‐dependent ATPase. Their interaction could significantly enhance the ATPase activity of hPrp43 and induce a growth‐promoting effect on mammalian cells. Because northern blot analyses of normal human organs implied GPATCH2 to be a novel cancer/testis antigen, targeting GPATCH2 or inhibition of the interaction between GPATCH2 and hPrp43 could be a promising novel therapeutic strategy of breast cancer. ( Cancer Sci 2009)