Open AccessTranslocation t(11;18)(q21;q21) in gastric B‐cell lymphomas
Author(s) -
Toracchio Sonia,
Ota Hiroyoshi,
de Jong Daphne,
Wotherspoon Andrew,
Rugge Massimo,
Graham David Y.,
Samani Amir,
ElZimaity Hala M.T.
Publication year - 2009
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2009.01128.x
Subject(s) - lymphoma , chromosomal translocation , pathology , malt lymphoma , lymphatic system , mucosa associated lymphoid tissue , biology , b cell , gastric mucosa , fluorescence in situ hybridization , microbiology and biotechnology , stomach , medicine , immunology , antibody , gene , biochemistry , chromosome
Translocation t(11;18)(q21;q21) is the most frequent chromosomal aberration reported in gastric mucosa‐associated lymphoid tissue (MALT) lymphomas. Intriguingly, this translocation has been reported only rarely in diffuse large B‐cell lymphomas; it has been proposed that t(11;18)‐positive tumors rarely progress to diffuse large B‐cell lymphomas. We examined the frequency of chromosomal translocation t(11;18)(q21;q21) in mucosa‐associated lymphoid tissue lymphoma and diffuse large B‐cell lymphoma of the stomach. Paraffin‐embedded tissues from patients with gastric B‐cell lymphomas were selected retrospectively. The presence of the t(11;18)(q21;q21) was determined using reverse transcriptase–polymerase chain reaction and/or fluorescence in situ hybridization. β‐Actin transcript was also determined to evaluate the integrity and efficiency of RNA (cDNA) recovery from paraffin‐embedded tissues. We analyzed 53 gastric B‐cell lymphomas (33 diffuse large B‐cell and 20 mucosa‐associated lymphoid tissue) obtained from Italy, the USA, or Japan. β‐Actin transcript was amplified in 50 cases (94%), including 19 mucosa‐associated lymphoid tissue and 31 diffuse large B‐cell lymphomas (five with mucosa‐associated lymphoid tissue components). The t(11;18) translocation was detected in 19% (6 of 31) cases with diffuse large B‐cell lymphoma versus 26% (five of 19) with mucosa‐associated lymphoid tissue lymphoma ( P = 0.72). One of five diffuse large B‐cell lymphomas with a mucosa‐associated lymphoid tissue component showed the t(11;18)(q21;q21). In conclusion, translocation t(11;18)(q21;q21) was found in both mucosa‐associated lymphoid tissue lymphomas and diffuse large B‐cell lymphomas of the stomach at approximately equivalent frequencies; its presence does not exclude progression to diffuse large B‐cell lymphoma. ( Cancer Sci 2009; 100: 881–887)