Open AccessPolyinosinic‐polycytidylic acid liposome induces human hepatoma cells apoptosis which correlates to the up‐regulation of RIG‐I like receptors
Author(s) -
Peng Shuo,
Geng Jianlin,
Sun Rui,
Tian Zhigang,
Wei Haiming
Publication year - 2009
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2008.01062.x
Subject(s) - receptor , rig i , apoptosis , biology , microbiology and biotechnology , mda5 , stimulation , innate immune system , biochemistry , rna interference , endocrinology , rna , gene
Toll‐like receptor 3 and RIG‐I like receptors (RLRs; MDA5, RIG‐I) are involved in cell growth inhibition and apoptosis. However, the toll‐like receptor 3‐related apoptotic pathway is insensitive to direct polyinosinic‐polycytidylic acid (dsRNA analog) stimulation in hepatoma cells. To determine whether the strategy of transferring polyinosinic‐polycytidylic acid into cells (polyinosinic‐polycytidylic acid‐liposome) could induce apoptosis in hepatoma cells through cytoplasm receptors, we examined the responses of innate immune receptors RLRs and toll‐like receptor 3 in response to different stimulation. We found that the apoptosis could exclusively be detected under polyinosinic‐polycytidylic acid‐liposome stimulation, which involved the activation of the caspase pathway. Besides, the expression of RIG‐I, MDA5, IFNβ and interferon‐stimulated gene 15 was increased significantly at an early stage. Moreover, the growth inhibition of polyinosinic‐polycytidylic acid‐liposome was confirmed in a mouse model. Taken together, these results suggest polyinosinic‐polycytidylic acid‐liposome could be used as a potential apoptotic agent in hepatocellular carcinoma cells and imply a potential therapeutic strategy. ( Cancer Sci 2009; 100: 529–536)