Open AccessHSP70 and mucin 5B: novel protein targets of N,N ′‐dinitrosopiperazine‐induced nasopharyngeal tumorigenesis
Author(s) -
Tang FaQing,
Duan ChaoJun,
Huang DaMao,
Wang WeiWei,
Xie ChunLei,
Meng JingJing,
Wang Lei,
Jiang HaiYing,
Feng DeYun,
Wu ShangHui,
Gu HuanHua,
Li MoYu,
Deng FuLiang,
Gong ZhiJun,
Zhou Hui,
Xu YongHao,
Tan Can,
Zhang Xin,
Cao Ya
Publication year - 2009
Publication title -
cancer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.035
H-Index - 141
eISSN - 1349-7006
pISSN - 1347-9032
DOI - 10.1111/j.1349-7006.2008.01028.x
Subject(s) - hsp70 , carcinogenesis , nasopharyngeal carcinoma , mucin , western blot , blot , heat shock protein , microbiology and biotechnology , biology , chemistry , pathology , cancer research , medicine , cancer , radiation therapy , biochemistry , genetics , gene
N,N ′‐Dinitrosopiperazine (DNP) induces nasopharyngeal carcinoma (NPC) and shows organ specificity to the nasopharyneal epithelium. To investigate its mechanism, the rat NPC model was inducd using DNP. Rat NPC and normal nasopharyngeal cells were obtained from the NPC model using laser capture. The total proteins from these cell samples were separated with two‐dimension polyacrylamide gel electrophoresis techniques, and highly expressed proteins (> five‐fold) were analyzed using matrix‐assisted laser desorption/ionization time of flight and bioinformatics. The results showed that HSP70 and mucin 5B expression increased not only in rat NPC but also in atypical hyperplasia nasopharyngeal tissues, a precancer stage of NPC. High‐expression of heat shock protein 70 (HSP70) and mucin 5B was further supported by western blot analysis. The immunofluorescence and western‐blotting studies further showed that DNP induced the expression of HSP70 and mucin 5B in a dosage‐dependent manner in normal nasopharyngeal epithelia cells. Our data indicate that DNP triggers over‐expression of HSP70 and mucin 5B, and is involved in nasopharyngeal tumorigenesis. HSP70 and mucin 5B may be important targets in nasopharyngeal tumorigenesis induced by DNP. ( Cancer Sci 2009; 100: 216–224)